Evanescence Technology (EVA)
ELIONOVA's Real-Time immunoassay technology is based ton fluorescence waveguide laser evanescent illumination.
Key features of the technology are:
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High precision : The technology provides quantitative results comparable to high standard labratory instruments.
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Speed : Time-to-result are only 10 minutes.
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Labor : It is a wash-free method, requiring NO pipetting steps
How does the EVA Technology works?
EVA technology (ELIODX ® ) generates an evanescent field of 200nm depth at the bottom of the cuvette. Fluorophores in this field are excited and fluoresce. The exciting laser light beam (635nm) is totally reflected at the liquid-solid interface. Photons of fluorescence signal are recorded by a photomultiplier. Most of unbound fluorophore is not excited hence not giving a signal except some background. Fluorophores bound in the antibody sandwich are captured in the evanescent field and signal increases overtime. The slope of the generated signal is proportional to the analyte concentration.
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